Did my IP work? Where is my signal? How well do my replicates correlate? What might my peaks even look like? Where are my peaks (or signal) in relationship to transcription start sites (or other features)? These are common questions that biologists first pose when dealing with ChIPseq data. We will use deepTools and MACS within Galaxy to demonstrate effective methods of
- performing ChIPseq-specific quality control,
- calling peaks and
- visualising signal and peak enrichment around genes or other features.
Prerequisites
- Galaxy 101 or equivalent experience.
- Ideally participants will already be familiar with generic NGS quality control and read mapping, since those won't be covered
- A wi-fi enabled laptop with a modern web browser. Google Chrome, Firefox and Safari will work best.
