➔ Slides (doi: 10.7490/f1000research.1114389.1)
This workshop will focus on visualisation of large datasets using the built-in tools of Galaxy, focusing on primary next-generation sequencing (NGS) data and the resulting downstream, aggregated data. First, using a multi-omic dataset consisting of exome and transcriptome (RNA-seq) data, participants will visualise alignments, variation, expression levels, and annotations using the Galaxy’s built-in genome browser, Trackster. Participants will learn how to create a genome visualisation, add data, configure data, move between a linear genome browser view and a Circos view, and generate complex genome visualisations (figures) with more than 12 NGS datasets. Second, using a processed multi-omic dataset, participants will create a several numerical plots (e.g., scatter plot, histogram) to gain an overview of the data. Based on insight gained from these visualisations, participants will create a heatmap to identify patterns and potential causal factors. All visualisations will be created, saved, and shared using only Galaxy and a web browser; no data or software uploads or downloads will be necessary.
