➞ Slides
This workshop will introduce the concepts behind transcriptomics with NGS data and how to analyze this data in Galaxy. Specifically, this workshop will focus on de novo transcriptome reconstruction of RNA-seq data with the following goals:
- comprehensive identification of all transcripts across an experiment
- appropriately annotating classes of transcripts
- generating abundance estimates across a transcriptome
- significance testing of differentially expressed transcripts
- visualisation of reads and transcript structures with Trackster
Prerequisites
- a general knowledge of Galaxy (for example, you should be familiar with the material in Galaxy 101 or have attended Introduction to Galaxy).
- a wi-fi enabled laptop with a modern web browser. Google Chrome, Firefox and Safari will work best.